Part:BBa_K112101:Design

Cre enzyme without stop codon

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 42
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

This part is built in Biobrick version 2.0. In this format, parts are flanked by BglII and BamHI restriction sites.

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PCR mv003/mv004 on pBca9145-Bca1010    (1060bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112101
-----------------------------------------------
mv003	Forward Biobricking of Cre>
ccataGAATTCatgAGATCTatgtccaatttactgaccgtac
mv004	Reverse Biobricking of Cre>
cgttaGGATCCatcgccatcttccagcaggcg

Source

PCR mv003/mv004 on pBca9145-Bca1010    (1060bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112101